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Alongside their use as ‘synthetic antibodies’ for detection, nanoMIPs can also be used to simplify workflows and introduce process improvements in the IVD sector. Their high affinities, specificities and robust characteristics make them ideal candidates for sample preparation, protein enrichment, and interference removal. The ability to ‘build-in’ certain functionalities also means nanoMIPs can eliminate entire workflow segments such as label conjugation or introduction of magnetic features. Sample preparation Patient samples and other biological solutions often require a level of purification to remove contaminants or to enrich the target of interest prior to analysis. Sample preparation techniques can include centrifugation, precipitation, electrophoresis and chromatography techniques. nanoMIPs are ideal for chromatography techniques, due to their high specificities and affinities, along with other unique benefits: Perfect for protein enrichment of low concentration biomarkers nanoMIPs can be used in affinity chromatography to bind proteins of high abundance when the target biomarker is in a low concentration. The high abundance proteins remain on the column and the target biomarker is eluted in a higher concentration, prior to analysis. Can withstand high temperatures or pH during purifications nanoMIPs have been shown to withstand high temperatures and pH, which makes them ideal for high throughput purifications in which temperature or pH are used as an elution step. Selectable affinities to suit the application The nanoMIP development process allows for an element of control over the affinity of the nanoMIP. This allows for reusable columns for purification if required. Interference removal IVD assay signal response interference can cause false positive or false negatives and is therefore a significant concern when developing a new assay. Typical interference can include structurally similar biomarkers, heterophile antibodies, human anti-animal antibodies, rheumatoid factors and other proteins in the sample. Some of the most efficient methods of removing interference is through the extraction of the specific analyte from the sample using chromatographic techniques or immunoextraction. nanoMIPs are applicable to both these techniques, and can be immobilised on a column, or added directly to the solution. They also work as interference blockers when directly incorporated into the immunoassay, binding the interfering analyte and ‘blocking’ it from binding the capture or detection antibody. Not only are they highly specific to the interfering analyte, nanoMIPs offer a range of other unique benefits for interference removal: High affinity to the interference target The nanoMIP development method enables an element of control over the affinity which means it can be optimised for the specific end use. Can withstand high temperature and pH nanoMIPs have been proven to withstand high temperatures and pH, making them ideal for any heat treatments for interference removal where the diagnostic marker is heat stable. Highly specific Since nanoMIPs are developed around the target analyte, they are highly specific, and can differentiate between molecules that are structurally similar. Get in touch to see how your workflow can be simplified